Fig. 6: CRH induces mitochondrial fission.
A Graphical representation of mitochondrial dynamic: fusion and fission with relative proteins (OMM = outer mitochondrial membrane; IMM = inner mitochondrial membrane). Primary hippocampal neurons at DIV14 were treated with CRH 100 nM for 0.5 h and with CRH 0.5 h + CRHR1 Blocker NBI30775 100 nM; Western Blot analysis and quantification of B the fission proteins DRP1S616, C FIS1 and the fusion markers D OPA1, E MFN1 and F MFN2. G Summary of the western blot results. Experiments were performed in N = 3 independent replicates at DIV14. Data are displayed as Mean ± SEM; one-way ANOVA and Bonferroni’s post hoc comparison test were performed; data non normally distributed were analyzed by Kruskal–Wallis non parametric test followed by Uncorrected Dunn’s multiple comparison test. (*p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001).
