Table 1 Sequences utilized in this study.
qPCR primers | ||
|---|---|---|
Genes | Forward (5′–3′) | Reverse (5′–3′) |
BRDT | AAGCCTCCTCTGAAGGGAACTC | GGGACAAAACCTGGAGCTGTTG |
PLK1 | GCACAGTGTCAATGCCTCCAAG | GCCGTACTTGTCCGAATAGTCC |
AURKC | CCACAGTGAGACTTACAGACGC | GCTGGTATCTGAGAAGCCTGGA |
GAPDH | GTCTCCTCTGACTTCAACAGCG | ACCACCCTGTTGCTGTAGCCAA |
shRNA sequences (5′–3′) | ||
Scramble shRNA | UAGCGACUAAACACAUCAATT | |
BRDT shRNA-1 | AAGACTTCAATACAATGTTCTCA | |
BRDT shRNA-2 | AAGATGAGCGAGTTAAGCGTCTT | |
BRDT shRNA-3 | AATAATGATGTCCAAAGAAGAAC | |
PLK1 shRNA | GTGCTTCGAGATCTCGGAC | |
AURKC | CCACGATAATAGAGGAGTTGGCAGATGCC | |
sgRNA sequences (5′–3′) | ||
BRDT sgRNA-1 | TCTCCCTTGAACGTGGTACA (Target DNA Sequence) | |
BRDT sgRNA-2 | TCTCCCTTGAACGTGGTACA (Target DNA Sequence) | |
