Fig. 4: circXPO1 binds to IGF2BP1 and enhances CTNNB1 mRNA stability.
From: A novel circular RNA, circXPO1, promotes lung adenocarcinoma progression by interacting with IGF2BP1
(a) Subcellular location of circXPO1 in A549 cells. (b) Flow chart of the MS2 pull-down assay protocol. (c) RNA immunoprecipitation assay using the anti-IGF2BP1 antibody in A549 cells. lncRNA THOR is used as a positive control of IGF2BP1-binding RNA. Canonical target mRNAs of IGF2BP1 were detected by qRT-PCR after silence (d) and overexpression of (e). qRT-PCR was used to measure the expression levels of CTNNB1 after actinomycin D treatment in the context of circXPO1 overexpression (f) and (g) silence in A549 cells. (h) Protein expression of circXPO1 downstream targets upon silence and overexpression in A549 cells. (P values were calculated by non-paired student tests. *P < 0.05, **P < 0.01. The error bars indicate the standard deviations.).
