Fig. 3: STING signaling promotes mtDNA-mediated necroptosis. | Cell Death & Disease

Fig. 3: STING signaling promotes mtDNA-mediated necroptosis.

From: mtDNA-STING pathway promotes necroptosis-dependent enterocyte injury in intestinal ischemia reperfusion

Fig. 3: STING signaling promotes mtDNA-mediated necroptosis.

(A) Representative images of intestinal H&E, IHC (p-MLKL), and TUNEL staining in STING knockout mice after the intraperitoneal injection of mtDNA. (B) Plasma IFN-β and IL-6 were tested by ELISA. (C) STING signaling and necroptosis were analyzed by western blot. (D) Primary intestinal epithelial cells (IECs) isolated from wild-type or STING knockout mice were stimulated with 50 μg/ml DMXAA or 10 μg/ml mtDNA. Western blot was used to analyze STING and necroptosis signaling. (E) Primary IECs were treated with 10 μg/ml mtDNA, 1 μM IFN alpha-IFNAR-IN-1 (IFNAR-IN) or 2 μM SPD304. Western blot was used to analyze necroptosis signaling. Scale bars = 50 μm. Data were shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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