Fig. 2: Modeling different levels of TRPM8 in RWPE-1 and LNCaPFGC prostate cell lines. | Cell Death & Disease

Fig. 2: Modeling different levels of TRPM8 in RWPE-1 and LNCaPFGC prostate cell lines.

From: Calcium cytotoxicity sensitizes prostate cancer cells to standard-of-care treatments for locally advanced tumors

Fig. 2

a Western blot analysis and quantification of full-length 6TM TRPM8 amount in RWPE-1 and LNCaPFGC cell lines expressing endogenous (WT), overexpressed (M8) or knocked-out (CAS) levels of the protein. b Immunofluorescence analysis and quantification of PM-associated full-length 6TM TRPM8 in RWPE-1 and LNCaPFGC cell lines with endogenous (WT), overexpressed (M8), or knocked-out (CAS) levels of the channel. For quantification of PM TRPM8 positive cells a total of 6000 cells were counted from different fields. Scale bar, 5 μm. c–e Morphology (c), growth (d), and cell death (e) analyses of RWPE-1 and LNCaPFGC cell lines with endogenous (WT), overexpressed (M8), or knocked-out (CAS) levels of TRPM8. Error bars, mean ± SD. Experiments were performed in triplicate; data were analyzed using a two-tailed Student’s t-test. **P ≤ 0.01; ***P ≤ 0.001.

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