Fig. 3: Con A-induced inflammation was suppressed in hCNR1−/− mice. | Cell Death & Disease

Fig. 3: Con A-induced inflammation was suppressed in hCNR1−/− mice.

From: Hepatocyte cannabinoid 1 receptor nullification alleviates toxin-induced liver damage via NF-κB signaling

Fig. 3: Con A-induced inflammation was suppressed in hCNR1−/− mice.

hCNR1+/+ and hCNR1−/− mice were injected with Con A (5 mg/kg) for 12 h (n = 6). A Blood samples were collected post-Con A injection (5 mg/kg) after 12 h period (n = 3–6) and measured proinflammatory cytokines, IL-6, IL-1β, IL-17, TNF-α, and IFN-γ. B Protein extracts from liver homogenates were immunoblotted and detected by autoradiographic signals. The bands were subjected to densitometric quantifications for protein expression levels of total p65, phospho-p65 (p-p65), and IKKβ (n = 4). C Total p65 and p-p65 nuclear and cytoplasmic protein amounts in the liver lysates. Nuclear and cytoplasmic fractions were normalized to lamin A/C and GAPDH, respectively. Results were expressed as mean ± S.E.M. (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 compared to hCNR1+/+ mice).

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