Fig. 2: Decreased neuronal markers in NPC organoids. | Cell Death & Disease

Fig. 2: Decreased neuronal markers in NPC organoids.

From: Human iNSC-derived brain organoid model of lysosomal storage disorder in Niemann–Pick disease type C

Fig. 2: Decreased neuronal markers in NPC organoids.

a Clearing and immunostaining with DAPI in the WT and NPC organoids at day 28. Scale bars, 500 μm. b Immunostaining for mitotic radial glia (phospho-vimentin) in the outer region. Scale bars, 50 μm. c Staining for neuronal markers (TUJ1, NF, and MAP2) in the WT and NPC organoids at day 28. Scale bars, 50 μm. d The percentage of the total number of cells that expressed TUJ1, NF, and MAP2 of the total number of DAPI-positive cells counted in each group was determined. e, f Western blot analysis and quantification of neuronal markers (TUJ1, NF, and MAP2) reveals a reduction in the NPC organoids on day 28. Every experiment was performed in triplicate. The results are the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001.

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