Fig. 5: SK1 knockdown does not affect major components of the senescence-associated secretory phenotype induced by oncogenic K-Ras overexpression. | Cell Death & Disease

Fig. 5: SK1 knockdown does not affect major components of the senescence-associated secretory phenotype induced by oncogenic K-Ras overexpression.

From: Targeting sphingosine kinase 1 (SK1) enhances oncogene-induced senescence through ceramide synthase 2 (CerS2)-mediated generation of very-long-chain ceramides

Fig. 5

A–D Analysis of expression of cytokines and chemokines at mRNA levels by RT-qPCR in cells overexpressing Vect or GV K-Ras that have been transfected with control (AS) or SK1 siRNA (20 nM) for 72 h. TaqMan probes for IL-6, IL-8, IL-1, and IL-12 were used and Actin was used as a control gene. The mean normalized expression of each mRNA was determined by using the Ct values (N = 3, data are presented as mean ± SEM, two-way ANOVA with Tukey’s multiple comparisons test, * means p < 0.05 comparing the group tested to Vector control siRNA, # means p < 0.05 comparing the group tested to GV control siRNA).

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