Fig. 3: Sodium current (I_Na) and action potentials (AP) of cFat10^−/− cardiomyocytes.

A, B Peak I_Na in ventricular myocytes isolated from cFat10^−/− and Fat10^fl/fl mice. Representative traces of I_Na with a pulse protocol (A) and the current–voltage relationship of I_Na (B) are shown (n = 13 for Fat10^fl/fl cells and n = 15 for cFat10^−/− cells). C, D Late sodium current (I_Na,L) was measured in response to 200 ms voltage steps to −20 mV from a holding potential of −120 mV (mean I_Na,L at 150 ms, *p < 0.05, Fat10^fl/fl vs. cFat10^−/−). E, F I_Na,L in wild-type (Fat10^+/+) HEK293 cells (n = 11) or Fat10^−/− HEK293 cells (n = 18) expressing wild-type Scn5a. Representative traces of I_Na,L with a pulse protocol (E) and the current–voltage relationship of I_Na (F). (*p < 0.05, **p < 0.01, Fat10^+/+ vs. Fat10^−/− HEK293 cells). G, H I_Na,L was measured in response to 200 ms voltage steps to −20 mV from a holding potential of −120 mV (mean I_Na,L at 150 ms, *p < 0.05, Fat10^+/+ vs. Fat10^−/− HEK293 cells). I Representative APs were recorded from Fat10^fl/fl (n = 14) and cFat10^−/− (n = 11) ventricular myocytes. J–L AP duration (J), velocity of the AP upstroke (K), and AP amplitude (L) in ventricular myocytes isolated from cFat10^−/− mice or Fat10^fl/fl mice (*p < 0.05, **p < 0.01).