Fig. 4: Exo-STIM1-KO suppresses human umbilical vein endothelial cells (HUVEC) tube formation and increases miR-145 level.
From: STIM1 promotes angiogenesis by reducing exosomal miR-145 in breast cancer MDA-MB-231 cells
A Expression of STIM1 and phosphorylation of ERK in SKF96365-treated MDA-MB-231 cells. B Expression of STIM1 in STIM1-KO MDA-MB-231 cells. C Representative time-course recording of intracellular Ca2+ fluorescence in STIM1-KO-MDA-MB-231 cells. D Representative electron microscopic image of Exo-STIM1-KO. E The markers of exosomes in Exo-STIM1-KO, as determined by western blot. F The amount of Exo-STIM1-KO showed no significant change compared with Exo. G The uptake of Exo-STIM1-KO in HUVECs. HUVECs were cultured with PKH26-labeled Exo-STIM1-KO. H Tube formation was inhibited by Exo- STIM1-KO. I Quantification of the results in (H). J Relative levels of miRNAs in STIM1-silencing MDA-MB-231 cells. K The levels of miR-145 and miR-449 in Exo-STIM1-KO. L The levels of miR-145 and miR-449 in HUVECs after treatment with Exo and Exo-STIM1-KO. Data represent mean ± SEM from three independent experiments (n = 3). *P < 0.05, **P < 0.01.
