Fig. 5: AR nuclear translocation contributed to cell autophagic cell death. | Cell Death & Disease

Fig. 5: AR nuclear translocation contributed to cell autophagic cell death.

From: Suppressing BCL-XL increased the high dose androgens therapeutic effect to better induce the Enzalutamide-resistant prostate cancer autophagic cell death

Fig. 5

A Morphology shows that most EnzR-C4-2 cells were balled up after 50 nM DHT treatment. B–C RealTime-Glo™ Annexin-V Apoptosis and Necrosis Assay were performed to show Apoptosis (B) and Necrosis (C) after 50 nM DHT or H2O2 in EnzR-C4-2 cells. D–E Western blotting was performed on EnzR-C4-2 cell line to show that caspase-dependent apoptosis marker is decreased (D) and autophagy marker is increased (E) with 50 nM DHT treatment. F Transmission electron microscopy showed the status of autophagosomes (red arrows) after 50 nM DHT treatment. G–H Cell colony assay shows cell growth with 50 nM DHT when sh-Beclin-1 in EnzR-C4-2 (G) and EnzR-C4-2B (H) cell lines. I Western blotting shows LC3 expression after sh-Beclin-1 with 50 nM DHT. Data are presented as means ± SD. *p < 0.05 was considered statistically significant by students’ t-test for two groups or ANOVA for more than two groups.

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