Fig. 5: Increase in toxic effect of chloroquine and hydroxychloroquine in eif2α mutant cells. | Cell Death & Disease

Fig. 5: Increase in toxic effect of chloroquine and hydroxychloroquine in eif2α mutant cells.

From: Lysosomotropic agents including azithromycin, chloroquine and hydroxychloroquine activate the integrated stress response

Fig. 5

Human osteosarcoma U2OS either WT, ATG5−/−, TFEB−/−, TFE3−/−, TF DKO, PERK−/− or carrying an eIF2αS51A/S51A knockin mutation were treated with 10, 20, or 40 μM of chloroquine (CQ) or hydroxychloroquine (HCQ) for 24 h. Plasma membrane integrity loss and phosphatidylserine (PS) exposure (with) were measured by flow cytometry employing DAPI and AlexaFluor 647-coupled annexin V, respectively. DAPI+ and Annexin V+ DAPI− cellular populations were quantified and are depicted as a heatmap A. Data are means ± SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05, ##P < 0.01, ###P < 0.001 vs. WT, Student’s t-test). Data for WT and eIF2αS51A expressing mutant U2OS are depicted as bar chart in B. Staurosporine (STS) at 2 μM was used as a positive control for cell death induction. Data are means ± SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05, ##P < 0.01, vs. vehicle control (Ctrl), Student’s t-test).

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