Fig. 2: Circadian expression of clock genes in control and CCl4-treated mice.
From: Period1 mediates rhythmic metabolism of toxins by interacting with CYP2E1
A single intraperitoneal injection of CCl4 (0.6 mL/kg body weight) was administrated to WT at ZT1. Mice were killed at ZT1, ZT5, ZT9, ZT13, ZT17, ZT21, and ZT24. Hepatic mRNA levels of Bmal1 A, Clock B, Per1 C, Per2 D, Cry1 E, and Cry2 F were measured by real-time PCR after CCl4 treatment. CK: control. Solid lines indicate experimental response curves; dashed lines, fitted model curves. Red dashed line with the number represents mesor. Data were shown as means ± S.E.M. N = 5 independent biological replicates per group. Data represent cumulative results from three independent experiments.
