Fig. 5: Effect of Per1 deficiency on Cyp2e1 expression exposed to CCl4.

Liver tissues were collected 24 h after single CCl₄ administration. Hepatic mRNA levels of A Gclc, B Ho-1, C Cat, D Sod1, E Sod2, and F Cyp2e1 were measured by real-time PCR. CK: control. Data were shown as means ± S.E.M. N = 5 independent biological replicates per group. * indicates P value < 0.05, ** indicates P value <0.01, Per1^−/−group versus WT group; ^# indicates P value <0.05, ^## indicates P value <0.01, CCl₄ group versus control group. G Cyp2e1 expression was measured by real-time PCR in the mouse liver at ZT1 and ZT13. H Representative western blots for rhythm of CYP2E1 protein expression in the mouse liver. Extracts were measured via western blot analysis with anti-CYP2E1 or anti–β-ACTIN antibody. CK: control. Data were shown as means ± S.E.M. N = 3 independent biological replicates per group. * indicates P value <0.05, ** indicates P value <0.01, Per1^−/− group versus WT group; ^# indicates P value <0.05, ^## indicates P value <0.01, ZT1 group versus ZT13 group. I Western blot assay of CYP2E1 protein after CCl₄ treatment in vivo. J Representative images of immunohistochemical staining were shown for liver sections stained with anti-CYP2E1 antibody. This is in agreement with the RT-PCR and western blot data. Bar = 100 μm. Representative images from N = 3 biological replicates. CK: control. Data were shown as means ± S.E.M. N = 3 independent biological replicates per group. * indicates P value <0.05, ** indicates P value <0.01, Per1^−/− group versus WT group; ^# indicates P value <0.05, ^## indicates P value <0.01, CCl₄ group versus control group. Data represent cumulative results from three independent experiments.