Fig. 1: BTF3 plays an oncogenic role in prostate cancer in vitro and in vivo.
a BTF3 expression levels in prostate cancer and normal tissue. Transcriptome data of prostate tumors from TCGA and normal tissues from TCGA and GTEx samples were obtained from the UCSC Xena (https://xena.ucsc.edu). The gene expression is reported as log2(Transcripts per million (TPM) + 0.001). Each dot represents an individual sample (n = 496 for prostate tumor; n = 149 for normal tissue. The black lines in each group indicate the mean ± S.D. ****p < 0.0001 (Mann–Whitney test). b Immunohistochemistry staining of tissue microarray from prostate tumors and adjacent noncancerous tissues using anti-BTF3 antibody. Representative images of BTF3 stained tumor and their corresponding noncancerous tissue sections are shown in weak, Intermediate, and strong staining, respectively. The scatter plot graph showing a statistical analysis of BTF3 expression (H score) in tumor and adjacent noncancerous tissues. Data are shown as mean ± S.D.; n = 82 for tumors and n = 55 for adjacent noncancerous tissues. Scale bar, 25 μm. **p < 0.01 (Mann-Whitney test). c Relative cell growth of PC-3 and DU145 prostate cancer cells with or without BTF3-knockdown was measured by crystal violet assay. Data are shown as mean ± S.D. for three independent experiments. ***p < 0.001, ****p < 0.0001 (Student’s t-test). d, e DU145-Tet-On-shBTF3 xenografted mice were treated with or without doxycycline. The graph shows the fold change in tumor volume, with respect to the initial treatment at day 0 (d). The tumor volume and representative images of dissected tumors at the endpoint of treatment are shown (e). Dox, Doxycycline (2 mg/ml in drinking water). The data are shown as mean ± S.E.M (n = 6 for each group). ****p < 0.0001 (Student’s t-test).
