Fig. 3: Autophagosomes were induced in HER2-positive GC cells by T-DM1 treatment. | Cell Death & Disease

Fig. 3: Autophagosomes were induced in HER2-positive GC cells by T-DM1 treatment.

From: Targeting the autophagy promoted antitumor effect of T-DM1 on HER2-positive gastric cancer

Fig. 3: Autophagosomes were induced in HER2-positive GC cells by T-DM1 treatment.

A NCI-N87 cells were incubated with T-DM1 for 48 h. The levels of SQSTM1 and LC3-II were analyzed by immunoblot analysis. ImageJ software was employed to estimate densitometric values and to normalize the densitometric values to the corresponding control value. The results of control were set as 1.0, and the data are presented as the mean ± S.D. of 3 independent experiments. Student t-test was used to compare the data. *P < 0.05, **P < 0.01, and ***P < 0.001. B NCI-N87 cells were interfered with T-DM1 for 24 h, and TEM was used to detect ultrastructural autophagosomes shown in the electron photomicrograph. c Autophagic flux and lysosomes in NCI-N87 cells treated with T-DM1 for specific times were evaluated by Cyto-ID Green staining and LysoTracker Red staining.

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