Fig. 4: NORAD was associated with DNA damage repiar response and induced by H3K27ac. | Cell Death & Disease

Fig. 4: NORAD was associated with DNA damage repiar response and induced by H3K27ac.

From: Oxidative stress activates NORAD expression by H3K27ac and promotes oxaliplatin resistance in gastric cancer by enhancing autophagy flux via targeting the miR-433-3p

Fig. 4: NORAD was associated with DNA damage repiar response and induced by H3K27ac.

A, B Western blot to test the DNA damage repair related genes expression in SGC-7901 and KATO III: DNA-PKcs 450 kDa, ATR 300 kDa, Phospho-ATR (Ser428) 300 kDa, ATM 350kDA, Phospho-ATM (Ser1981) 350 kDa, H2AX 15 kDa, Phospho-Histone H2AX (Ser139) 15 kDa, β-Tubulin 55 kDa, GAPDH 37 kDa. C UCSC genome browser prediction about the enrichment of H3K27ac at the promoter region of NORAD in different cell lines. D NORAD location in SGC-7901, SGC-7901-R, KATO III, and KATO III-R by FISH tests. E H3K27ac expression in SGC-7901, SGC-7901-R, KATO III, and KATO III-R by western blot: H3K27ac 17 kDa, Histone H3 17 kDa. F H3K27ac location in SGC-7901, SGC-7901-R, KATO III, and KATO III-R by FISH tests. G CREBBP expression in SGC-7901, SGC-7901-R, KATO III, and KATO III-R by western blot: CREBBP 300 kDa, β-Actin 45 kDa. H CREBBP location in SGC-7901, SGC-7901-R, KATO III, and KATO III-R by FISH tests. I C646 can decrease the expression of NORAD in SGC-7901, SGC-7901-R, KATO III, and KATO III-R (SGC-7901, t = 11.52, P = 0.000; SGC-7901-R, t = 18.28, P = 0.000; KATO III, t = 17.64, P = 0.000, KATO III-R, t = 33.85, P = 0.000). J ChIP results to test the enrichment of H3K27ac at the promoter region of NORAD in SGC-7901, SGC-7901-R, KATO III, and KATO III-R. anti-H3k27ac vs anti IgG: SGC-7901, t = 17.19, P = 0.000; SGC-7901-R, t = 21.03, P = 0.000; KATO III, t = 31.22, P = 0.000, KATO III-R, t = 20.82, P = 0.000. K ChIP results to test the enrichment of CREBBP at the promoter region of NORAD in SGC-7901, SGC-7901-R, KATO III, and KATO III-R. anti-H3k27ac vs anti IgG: SGC-7901, t = 9.83, P = 0.000; SGC-7901-R, t = 15.83, P = 0.000; KATO III, t = 7.99, P = 0.000, KATO III-R, t = 19.92, P = 0.000. L Downregulation of CREBBP in SGC-7901-R and KATO III-R: CREBBP 300 kDa, β-Tubulin 55 kDa. M CREBBP knockdown can inhibit the enrichment of H3K27ac at the promoter region of NORAD in SGC-7901-R and KATO III-R. anti-H3k27ac: SGC-7901 vs SGC-7901-R, t = 16.23, P = 0.000; KATO III vs KATO III-R, t = 13.79, P = 0.000.

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