Fig. 7: miR-433-3p regulated autophagy by targeting ATG5 and ATG12. | Cell Death & Disease

Fig. 7: miR-433-3p regulated autophagy by targeting ATG5 and ATG12.

From: Oxidative stress activates NORAD expression by H3K27ac and promotes oxaliplatin resistance in gastric cancer by enhancing autophagy flux via targeting the miR-433-3p

Fig. 7

A Expression of ATG5 and ATG12 in miR-433-3p upregulated SGC-7901-R and KATO III-R cell line: ATG5(ATG5-ATG12 complex) 55 kDa, ATG12(free ATG12) 16 kDa, GAPDH 37 kDa. B Expression of ATG5 and ATG12 in miR-433-3p knockdown SGC-7901-R and KATO III-R cell line: ATG5(ATG5-ATG12 complex) 55 kDa, ATG12(free ATG12) 16 kDa, ATG 78 kDa, GAPDH 37 kDa. C Dual-luciferase reporter gene assay for miR-433-3p and ATG12 in SGC-7901-R (t = 8.789, P < 0.000) and KATO III-R (t = 15.29, P < 0.000). D Dual-luciferase reporter gene assay for miR-433-3p and ATG5 in SGC-7901-R (t = 2.260, P = 0.105) and KATO III-R (t = 7.296, P = 0.002). E Expression of ATG5 and ATG12 in NORAD-Wt(containing the binding site for miR-433-3p) upregulated SGC-7901-R and KATO III-R cell line: ATG5(ATG5-ATG12 complex) 55 kDa, ATG12(free ATG12) 16 kDa, ATG 78 kDa, GAPDH 37 kDa. F Expression of ATG5 and ATG12 in NORAD-Mut (containing the binding site for miR-433-3p) upregulated SGC-7901-R and KATO III-R cell line: ATG5(ATG5-ATG12 complex) 55 kDa, ATG12(free ATG12) 16 kDa, ATG 78 kDa, GAPDH 37 kDa. G miR-433-3p knockdown could reverse the downregulation of ATG12 and ATG5 that was induced by NORAD knockdown. Group A: NORAD knockdown + miR-433-3p knockdown; Group B: NORAD knockdown; Group C: miR-433-3p knockdown.

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