Fig. 4: CRC cell-derived exosomal CRNDE-h increased the expression and activity of RORγt. | Cell Death & Disease

Fig. 4: CRC cell-derived exosomal CRNDE-h increased the expression and activity of RORγt.

From: Tumor exosome promotes Th17 cell differentiation by transmitting the lncRNA CRNDE-h in colorectal cancer

Fig. 4

A Naive CD4+ T cells treated with CRC cell-exosome (SW480-E, HT29-E, and LOVO-E) for 72 h, and then the protein level of RORγt was detected by western blot. B Naive CD4+ T cells treated with exosome derived by CRC cells (SW480, HT29, and LOVO) were transfected with si-CRNDE-h (or si-control) for 72 h. Later, the protein level of RORγt was detected by western blot. C Jurkat cells were transfected with IL-17 promoter-driven luciferase vector, and then treated with CRC cell-exosome (SW480-E, HT29-E, and LOVO-E) for 72 h. Later, luciferase activity was detected. D Jurkat cells were transfected with IL-17 promoter-driven luciferase vector, and then treated with exosome derived by CRC cells (SW480, HT29, and LOVO) transfected with si-CRNDE-h (or si-control) for 72 h. Later, luciferase activity was detected. E: exosome. *p < 0.05, **p < 0.01 vs. NCM460-E or si-control-E.

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