Fig. 7: Constitutively active DRP1-S616E restores oxidative metabolism of NIK-deficient GBM cells.
From: NIK promotes metabolic adaptation of glioblastoma cells to bioenergetic stress
a Oxygen consumption rate was determined using the Seahorse Mito Stress test with control and NIK−/− cells (see Figs. 2b, 4a, b), as well as NIK−/− cells ectopically expressing DRP1-S616E that were cultured in 18 mM glucose (GLUC) or 18 mM galactose (GAL) media. Data depict the mean of ≥3 replicates per each cell type. b–d Individual mitochondrial function parameters were calculated from the data shown in a and shown as fold change compared to control cells in GLUC. b Basal respiration. Different letters indicate statistical significance using one-way ANOVA with Tukey post-hoc test. All comparisons have p < 0.0001. c ATP production. All statistical comparisons have p < 0.0001 except “a vs f” (p < 0.001). d Spare respiratory capacity. All statistical comparisons have p < 0.0001 except “a vs aa” (ns), “aa vs b” (p < 0.001), “aa vs d” (p < 0.05) “a vs c” (p < 0.001), and “c vs e” (p < 0.001).
