Fig. 4: NLRP3 inflammasome was inhibited by P2X7 receptor deficiency during renal ischemic-reperfusion injury. | Cell Death & Disease

Fig. 4: NLRP3 inflammasome was inhibited by P2X7 receptor deficiency during renal ischemic-reperfusion injury.

From: P2X7 receptor signaling promotes inflammation in renal parenchymal cells suffering from ischemia-reperfusion injury

Fig. 4

For A to D, male C57BL/6 wild-type mice and P2X7 receptor (−/−) counterparts were subjected to renal ischemic-reperfusion or sham operation (n = 5 per group). A The levels of NLRP3 mRNA and ASC mRNA by real-time polymerase chain reaction. B Representative immunoblots of whole-kidney lysate for the expression of NLRP3 inflammasome associated proteins (IL-1β p17, NLRP3, cleaved caspase-1, and ASC). C Representative immunofluorescent staining for IL-1β (green) and NLRP3 (red) merged with DAPI (blue), and D immunochemistry for cleaved caspase-1. For E and F, HK2 cells were subjected to ATP at 4 mM, and were transfected -or not- with negative control siRNA (Neg Con siRNA) or P2X7 receptor siRNA, or treated with A438079. E The levels of NLRP3 mRNA and ASC mRNA were measured by real-time polymerase chain reaction. F Representative immunoblots of cell lysate for the expression of NLRP3, ASC, cleaved caspase-1 proteins in response to ATP at 4 mM. A438079 was applied to block P2X7 receptor at 20 µM. *p < 0.05, **p < 0.01.

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