Fig. 3: ADT-OH further enhances the apoptosis induced by FADD. | Cell Death & Disease

Fig. 3: ADT-OH further enhances the apoptosis induced by FADD.

From: ADT-OH, a hydrogen sulfide-releasing donor, induces apoptosis and inhibits the development of melanoma in vivo by upregulating FADD

Fig. 3: ADT-OH further enhances the apoptosis induced by FADD.The alternative text for this image may have been generated using AI.

a Flow cytometric analysis of B16F10 cells treated with ADT-OH (2 μM) after transfection of pcDNA 3.1-FADD or pcDNA 3.1 vectors for 16 h. b Quantitative analysis of apoptosis as detected in a (mean ± SD, n = 3 independent experiments). c Detection of FADD expression levels by WB analysis after ADT-OH (2 μM) stimulation or transfection of FADD or empty plasmid in B16F10 cells. d Band intensity shown in c was quantified by Image J software. e Representative immunofluorescence staining for cleaved caspase-3 (green) after ADT-OH treatment and transfection of FADD or empty vectors for 16 h. f Relative expression levels of Bcl-2, Bax, Bad, cleaved-caspase 8 and cleaved-caspase 3 after ADT-OH stimulation or transfection of FADD or empty plasmid in B16F10 cells were detected by WB analysis using indicated antibodies. g Relative mRNA levels of Bcl-2, Bad and Bax after ADT-OH stimulation or transfection of FADD or empty plasmid in B16F10 cells were detected by qPCR analysis. *P < 0.05, **P < 0.01, compared with the control group.

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