Fig. 4: FADD is essential for ADT-OH-induced apoptosis. | Cell Death & Disease

Fig. 4: FADD is essential for ADT-OH-induced apoptosis.

From: ADT-OH, a hydrogen sulfide-releasing donor, induces apoptosis and inhibits the development of melanoma in vivo by upregulating FADD

Fig. 4: FADD is essential for ADT-OH-induced apoptosis.The alternative text for this image may have been generated using AI.

a Western blot analysis of FADD expression and mRNA levels of FADD in B16F10 transfection sg-nc and FADD KO cells after ADT-OH treatment. b B16F10 FADD KO cells were treated with ADT-OH at different concentration (0.8–50 μM) and apoptosis was determined by flow cytometry analysis (left). Quantitative analysis of apoptosis at various concentrations of exposure to ADT-OH (right). c Western blot analysis of FADD, Bad and Cleaved-Caspase 3 expression in A375 cells after ADT-OH treated in dose-dependent manner. d Detection of FADD expression levels by WB and qPCR analysis of FADD in A375 transfection si-NC and si-FADD interference fragment cells after ADT-OH treatment. e, f A375 control and interference FADD cells were treated with ADT-OH at different concentration (0.8–50 μM) and apoptosis was determined by flow cytometry analysis (e). Quantitative analysis of apoptosis at various concentrations of exposure to ADT-OH. Experiments (n = 3) were performed in triplicate (c, e). *P < 0.05, **P < 0.01, ****P < 0.001 compared with the vehicle group.

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