Fig. 3: TKT translocated into the nucleus and regulated the activity of the FXR promoter. | Cell Death & Disease

Fig. 3: TKT translocated into the nucleus and regulated the activity of the FXR promoter.

From: The nuclear translocation of transketolase inhibits the farnesoid receptor expression by promoting the binding of HDAC3 to FXR promoter in hepatocellular carcinoma cell lines

Fig. 3

a Luciferase activity analysis of the 2.5 kb FXR promoter at 48 h after the FXR promoter plasmid and empty vector or TKT-Flag expression plasmids were transfected into SMMC-7721 cells, Data are presented as mean ± squared error (SEM), the bar indicates the mean. Statistical significance was calculated using two-tailed unpaired t-test, *p < 0.05. b Nuclear and cytosolic fractionation analysis shows that TKT are localized in both the cytoplasm and nucleus of SMMC-7721 cells. Tubulin and PARP served as loading controls for the cytosolic and nuclear fraction, respectively. Every sample we loaded two lanes. c Immunofluorescent staining of endogenous TKT in SMMC-7721, SMMC-7721 cells with TKT shRNA and HepG2 cells.

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