Fig. 2: Overexpression of circFAM114A2 suppressed migration and invasion of UCB cells in vitro. | Cell Death & Disease

Fig. 2: Overexpression of circFAM114A2 suppressed migration and invasion of UCB cells in vitro.

From: Circular RNA FAM114A2 suppresses progression of bladder cancer via regulating ∆NP63 by sponging miR-762

Fig. 2

a The expression of circFAM114A2 was measured by qRT-PCR in 5637 and T24 after transfected overexpression plasmid. b–e The biological role of circFAM114A2 on cell migration, invasion and proliferation capability were assessed by wound healing, transwell migration, and matrigel invasion assay and CCK-8 assays in 5637 and T24. f The expression level of circFAM114A2 in 5637 and T24 cells after knocking-down by si-circFAM114A2. g–j The effect of si-circFAM114A2 on cell migration, invasion, and proliferation ability were assessed by wound healing, transwell migration, and matrigel invasion assays and CCK-8 assay in 5637 and T24. In wound healing assay, scale bars, 100 µm. In transwell assay, scale bars, 50 µm. Date are mean ± SEM, n = 3. ***p < 0.001, **p < 0.01, *p < 0.05 (student’s t test).

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