Fig. 3: circFAM114A2 serves as sponge of miR-762 in UCB cells. | Cell Death & Disease

Fig. 3: circFAM114A2 serves as sponge of miR-762 in UCB cells.

From: Circular RNA FAM114A2 suppresses progression of bladder cancer via regulating ∆NP63 by sponging miR-762

Fig. 3: circFAM114A2 serves as sponge of miR-762 in UCB cells.

a Schematic illustration showed the target miRNAs of circFAM114A2 predicted by RNAhybrid and miRanda. b The expression levels of four candidate miRNAs were detected by qRT-PCR in UCB cell lines. The level of miR-629-3P and miR-762 were increased, compared with SV-HUC-1. U6 was used as internal reference. c circFAM114A2 was pulled down by biotinylated probe from 5637 and T24 cells lysates, and detected by qRT-PCR. GAPDH was used as internal reference. d, e The expression levels of candidate miRNAs were assessed by qRT-PCR in circFAM114A2 pull-down products. GAPDH was used as internal reference. f RNA FISH showed that circFAM114A2 and miR-762 co-localized in cytoplasm. circFAM114A2 probe was labeled with cy3, miR-762 probe was labeled with FAM, and nuclei were stained with DAPI. Scale bars, 10 µm. Date are mean ± SEM, n = 3. ***p < 0.001, **p < 0.01, *p < 0.05 (student’s t test).

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