Fig. 3: PMAIP1 is a CK-resistant gene.
From: Genome-scale CRISPR screening for potential targets of ginsenoside compound K
a Read counts of sgRNAs targeting PMAIP1 in control and CK-treated groups. b Illustration of the sgRNA applied to deplete PMAIP1 in validation experiments. c Genome editing activity as assessed by T7E1 assay of sgRNA targeting PMAIP1. d Relative mRNA expression level of PMAIP1 in control and PMAIP1 sgRNA-treated cells. e Analysis of the protein level of PMAIP1 in control and PMAIP1 sgRNA-treated cells. f Representative images of cell state after CK (5 nM) treatment for 3 days. Scale bar = 150 µm. g Quantification of cell numbers in each cellular condition as presented in f. h Analysis of the LC3 protein level in control and PMAIP1 sgRNA-treated cells after CK treatment for 1 h. Data are represented as means with SEM. *P < 0.05, **P < 0.01.
