Fig. 1: Knockout of Ythdf2 in mouse spermatogonia cell line.
From: YTHDF2 promotes spermagonial adhesion through modulating MMPs decay via m6A/mRNA pathway
a Design of Ythdf2-sgRNA and DNA sequence of KO cells. Red letter represents target motif, green letter represents PAM. b Detection of YTHDF2 expression in WT cells and Ythdf2-KO cells by western blot. β-actin was used to eliminate the differences in loading amount. c Morphology of WT cells and Ythdf2-KO cells. Bar = 50 μm. d Cell viability of WT cells and Ythdf2-KO cells was detected using CCK-8 assay.
