Fig. 2: Electrophysiology and histology of paclitaxel-induced neuropathy in IL-6 knockout vs. wild-type mice.
From: Blockade of IL-6 signaling prevents paclitaxel-induced neuropathy in C57Bl/6 mice
a PTX treated WT mice developed a significant reduction of the sensory nerve action potential (SNAP) amplitude, whereas IL-6–/– mice receiving PTX showed no alterations in electrophysiological measurements. b Semiautomatic quantification of semi-thin sections of the sciatic nerve revealed a decrease in fiber density after PTX treatment in WT mice, while no changes could be detected in IL-6–/– mice after PTX therapy. c PTX treatment lead to a loss of larger myelinated fibers in WT mice, while IL-6–/– mice showed no shift in fiber distribution. d We observed a moderate positive correlation between mean axon diameter and SNAP amplitude. e Measurement of IL-6 concentrations in spinal cord lysates revealed significantly higher IL-6 concentrations in paclitaxel-treated wild-type mice (LLOD: Lower limit of detection). Error bars depict SEM. Statistical analysis: a, b two-way ANOVA with Tukey post hoc analysis of n = 10 mice/group, d Pearson correlation of n = 37 mice; e one-way ANOVA with Holm-Sidak post hoc analysis. *p < 0.05, NS not significant.
