Fig. 6: MRT199665 potentiates TGFβ-mediated apoptotic cell death.
From: Salt-inducible kinases (SIKs) regulate TGFβ-mediated transcriptional and apoptotic responses
a Immunoblot analysis of wild-type NMuMG murine mammary epithelial cells stimulated with TGFβ1 for the indicated durations. Cell lysates were resolved via SDS-PAGE, and membranes were subjected to immunoblotting with the indicated antibodies. b Immunoblot analysis of endogenous CRTC3 phosphorylation in wild-type NMuMG cells following incubation with DMSO, MRT199665 or HG-9-91-01. Cell lysates were subjected to CRTC3 IP and subsequently resolved via SDS-PAGE. Membranes were subjected to immunoblotting with the indicated antibodies. c Immunoblot analysis of wild-type NMuMG cells incubated with either SB-505124 (SB) or MRT199665 (MRT) in the presence of TGFβ1 stimulation. Cell lysates were resolved via SDS-PAGE, and membranes were subjected to immunoblotting with the indicated antibodies. d Immunoblot analysis of wild-type NMuMG cells incubated with MRT199665 in the presence or absence of TGFβ1 stimulation for the indicated durations. Cell lysates were resolved via SDS-PAGE, and membranes were subjected to immunoblotting with the indicated antibodies. e Annexin V staining analysis of wild-type NMuMG cells incubated with DMSO, SB-505124 or MRT199665 in the presence of TGFβ1 stimulation. Data represent the percentage of cells positive for annexin V staining from three independent experiments (5 × 104 cell counts per sample per replicate). f Crystal violet cellular viability analysis of wild-type NMuMG cells incubated with DMSO, SB-505124 or MRT199665 in the presence of TGFβ1 stimulation. Data represent cellular viability relative to unstimulated DMSO control cells.
