Fig. 4: Pyroptosis in primary cortical neurons.

a Representative scanning electron microscopy images in sham and oxyHb groups; b Western blot assay for the expression of GSDMD-N in the cell membranes; c Quantification of GSDMD-N; d Representative immunofluorescence staining images of AIM2, GSDMD, caspase-1 and ASC in control and oxyHb-exposed 24 h groups. Scale bars = 50 μm. White arrows show the pores caused by the N-terminal fragment of GSDMD. Bars represent the mean ± S.E.M. ***P < 0.001.