Fig. 1: IVIG induces autophagy in peripheral blood mononuclear cells. | Cell Death & Disease

Fig. 1: IVIG induces autophagy in peripheral blood mononuclear cells.

From: Intravenous immunoglobulin mediates anti-inflammatory effects in peripheral blood mononuclear cells by inducing autophagy

Fig. 1: IVIG induces autophagy in peripheral blood mononuclear cells.

a PBMCs from healthy donors were cultured with IVIG (10 or 25 mg/ml) or HSA for 24 h. Cells were treated with bafilomycin (Baf) for last 45 min. Levels of LC3-II and the fold changes in LC3-II levels (mean ± SEM, n = 4 donors) based on the densitometry analysis of western blots are presented. **P < 0.01; ***P < 0.001; ns not significant; by one-way ANOVA with Dunnett’s multiple comparison test. b Induction of autophagy flux by IVIG. PBMCs of healthy donors were cultured with IVIG (25 mg/ml) for 0, 18, or 24 h. At the end of treatment, cells were treated with or without Baf for 45 min. Autophagy flux (∆LC3-II) was quantified by subtracting the band intensity of LC3-II in the presence of Baf from that of absence of Baf. Representative blot of two different donors is shown.

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