Fig. 4: Subcellular localization of RD3 protein in murine retinas. | Cell Death & Disease

Fig. 4: Subcellular localization of RD3 protein in murine retinas.

From: GCAP neuronal calcium sensor proteins mediate photoreceptor cell death in the rd3 mouse model of LCA12 congenital blindness by involving endoplasmic reticulum stress

Fig. 4: Subcellular localization of RD3 protein in murine retinas.

Immunolocalization of RD3.V5 protein in retinal sections from rd3/rd3 mice electroporated with pRho-mRD3.V5-dsRed. RD3.V5 distributes between the inner and outer segment compartments of transfected photoreceptor cells, with a stronger signal at inner segments. Note that the expression of RD3.V5 in transfected cells restores RetGC1 expression levels and its transport to the outer segment. The red channel shows the dsRed signal from pRho-dsRed, a plasmid coelectroporated with pRho-mRD3.V5-dsRed to serve as a reporter in the identification of the eye injected area (Methods section). Anti-V5 antibody staining of the synaptic ribbons is observed in transfected and untransfected cells, and is an unspecific signal. Scale bar 20 μm.

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