Fig. 5: VDR knock out downregulated GPX4 and aggravate renal function loss, pathological changes, lipid peroxide peroxidation induced by cisplatin in mice model.
From: VDR activation attenuate cisplatin induced AKI by inhibiting ferroptosis
A, B Serum BUN and Cr levels of six groups of wild type and transgenic VDR knockout mice at 72 h after cisplatin injection. C Histological changes of renal cortex by HE staining at 72 h after cisplatin injection, and tissue injury scores in each group were statistically analyzed. Scale bar = 50 μm. D Renal cortex expression of VDR determined by immunohistochemical staining in wild type and VDR-KO mice after 72 h of cisplatin treatment. Scale bar = 50 μm. E Cortex expression of 4HNE determined by immunohistochemical staining after 72 h treatment of cisplatin, followed by semi-quantitative statistical analysis of each group. Scale bar = 50 μm. F Western blots analysis of GPX4 and VDR in renal tissue lysates from these four groups. The ratio of the optical density of GPX4 to β-actin was statistically analyzed. The data are presented as the mean ± SD, *P< 0.05, **P < 0.01. n = 8.
