Fig. 2: Different concentrations of TNF-α have opposite effects on cell fate. | Cell Death & Disease

Fig. 2: Different concentrations of TNF-α have opposite effects on cell fate.

From: The concentration of tumor necrosis factor-α determines its protective or damaging effect on liver injury by regulating Yap activity

Fig. 2

a TNF-α secretion in the livers of WT rats injected with LPS or APAP (n = 4 for each group). b, c Immunohistochemical staining of CD68+ Kupffer cells and numbers of positive cells in the livers of WT rats injected with LPS or APAP. Four liver sections were randomly selected from each group and used for immunohistochemistry analysis. Arrows indicates the CD68+ Kupffer cells. d Plasma ALT and AST levels in TNF-α−/− rats 12 h after injection of different concentrations of TNF-α (n = 4 for each group). e CCK-8 counts of BRL cells treated with different doses of TNF-α for 6 h (n = 6 for each group). f, g Cell death detection of BRL rat hepatocytes treated with different doses of TNF-α for 6 h using an Annexin V-FITC/PI double staining flow cytometry assay (n = 3 for each group). Data are expressed as the mean ± SD. *p < 0.05. Data are representative of at least three independent experiments.

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