Fig. 5: Different concentrations of TNF-α have different effects on the Yap activity of hepatocytes. | Cell Death & Disease

Fig. 5: Different concentrations of TNF-α have different effects on the Yap activity of hepatocytes.

From: The concentration of tumor necrosis factor-α determines its protective or damaging effect on liver injury by regulating Yap activity

Fig. 5

a Western blotting with the indicated antibodies of BRL cells treated with different doses of TNF-α for 6 h. b Immunoblot analysis of Yap1 in cytoplasmic (Cyt) and nuclear (Nuc) fractions of BRL cells after different treatments. c Immunofluorescence of the distribution of Yap1 in BRL cells after different treatments. Endogenous Yap1 (green) and nuclei (blue) were stained with specific antibody and DAPI, respectively. Arrowheads depict nuclear Yap1 expression in BRL cells. d Quantifications of Yap1 subcellular localization from at least 100 randomly selected cells in c. e Immunohistochemistry for Yap1 localization and expression in livers from rats treated with LPS or APAP and in normal rat liver. Arrowheads depict nuclear Yap1 expression in hepatocytes. f Quantifications of Yap1 subcellular localization from at least 200 randomly selected cells in e. C cytoplasm, N nucleus. Data are representative of at least three independent experiments.

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