Fig. 2: SIVA-1 is expressed in neurons during the development of the mouse brain. | Cell Death & Disease

Fig. 2: SIVA-1 is expressed in neurons during the development of the mouse brain.

From: SIVA-1 regulates apoptosis and synaptic function by modulating XIAP interaction with the death receptor antagonist FAIM-L

Fig. 2: SIVA-1 is expressed in neurons during the development of the mouse brain.

a At embryonic day 16 (E16), neurons in the cortical plate (CP) and subplate (SP) contained SIVA-1. Scale bar 100 μm. b At postnatal day 5 (P5), SIVA-1-immunopositive cells were located throughout all cortical layers. Scale bar 200 μm. c At postnatal day 15 (P15), SIVA-1-immunoreactive cells were present in all cortical layers. Note that cells located in layers II–III showed intense SIVA-1-immunolabeling. Scale bar 200 μm. d In the cerebral cortex of adult mice, expression of SIVA-1 was restricted to cortical cells in layer V. Scale bar 200 μm. e–g In the cerebellum, the soma and dendrites of Purkinje cells showed SIVA-1 immunostaining at all postnatal stages analyzed. Scale bar 200 μm. h In the hippocampus, pyramidal neurons and hippocampal interneurons expressed SIVA-1. Some hippocampal interneurons scattered in all hippocampal layers showed a strong immunostain (arrows in (h)). Scale bar 200 μm. i–k Double immunofluorescence of SIVA-1 and NeuN (arrows) in cortical layer V at P5, showing an almost complete co-localization of the two markers. Scale bar 25 μm. l–n Immunofluorescence co-localization of SIVA-1 and GFAP in cerebellum at P15 shows SIVA-1 expression in Purkinje cells (asterisks) but not in astrocytes (arrowheads) in the cerebellum. Scale bar 25 μm. o–q Immunofluorescence co-localization of SIVA-1 and PV shows expression of SIVA-1 in hippocampal interneurons (arrows). MZ marginal zone, IZ intermediate zone, SVZ subventricular zone, VZ ventricular zone, so stratum orines sp stratum pyramidale, sr stratum radiatum, ML molecular layer, PCL Purkinje cell layer, GL granule cell layer.

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