Fig. 3: Targeted DNA methylation of Mecp2 decreased the Mecp2 expression.
From: Locus-specific DNA methylation of Mecp2 promoter leads to autism-like phenotypes in mice
a Expression of Mecp2 in the hippocampus (left) and parietal cortex (right) of 18-week-old mice as analyzed with quantitative PCR. Methylation n = 4, control n = 3. b, c Western blot analysis and quantification of MeCP2 expression in hippocampus (b) and parietal cortex (c). Methylation n = 4, control n = 3. d Representative fluorescence images of MeCP2 (green) in the hippocampus and parietal cortex. The nuclei were co-stained with DAPI (blue). Scale bar, 100 µm. e Expression of MeCP2 was calculated by fluorescence intensity from 7 samples and 14 slides. Left: MeCP2 expression in the hippocampus; Right: MeCP2 expression in the parietal cortex. Methylation n = 4, control n = 3; Data were statistically analyzed by Student’s t test (*p < 0.05; **p < 0.01; ***p < 0.001), and shown as the mean ± s.e.m. The number of animals was indicated in the respective columns.
