Fig. 6: CYLD is crucial for RIPK1-FADD interaction and RIPK1 deubiquitination in MT4 cells. | Cell Death & Disease

Fig. 6: CYLD is crucial for RIPK1-FADD interaction and RIPK1 deubiquitination in MT4 cells.

From: Reversal of CYLD phosphorylation as a novel therapeutic approach for adult T-cell leukemia/lymphoma (ATLL)

Fig. 6

a Control or CYLD-deficient MT4 cells were treated for 16 h with a combination of MRT67307 and TPCA. Lysates were immunoprecipitated with anti-FADD and then sequentially blotted with anti-RIPK1 and anti-FADD. Equivalent portions of the lysates were also blotted as indicated in the lower panel. b Lysates from control or CYLD-deficient MT4 cells treated with kinase inhibitors for 8 h were immunoprecipitated with anti-RIPK1. Immune complex proteins were sequentially blotted with anti-ubiquitin and anti-RIPK1. Equivalent portions of the lysates were also blotted as indicated in the lower panel.

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