Fig. 5: 11C7 treatment inhibits inflammatory processes in injured retinal glia.

a, b Western blot analysis of whole retina lysates allowed to detect marked changes in the phosphorylation level of cofilin, P.Stat3 and P.Erk1/2 after NMDA/11C7 injection (n = 5 mice) compared with control eye treated with NMDA/control IgG (n = 4 mice). In its phosphorylated state, cofilin is inactivated by 11C7. In parallel, P.Stat3 downregulation suggests cytokine signaling reduction. c By immunofluorescence on retinal cross sections, P.cofilin upregulation was visualized in microglia/macrophages labeled with Iba1 (arrows). d P.Stat3 decrease was from Müller glia positive for glutamine synthetase (GS) and from isolectin B4 (IB4)-labeled blood vessels. e Following NMDA and 11C7 treatments, P.Erk1/2 immunoreactivity increased in the nucleus and in the endfeet (EF) of Müller cells identified with cytoplasmic GS. Immunofluorescent stainings were repeated in 3 mice/condition. Scales bars: c–e = 100 μm, close-up in d = 25 μm. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Statistics: *P < 0.05; **P < 0.01; Unpaired t-test.