Fig. 5: NR1D1 silencing activated MAPK and NF-κB signaling pathways.
From: NR1D1 modulates synovial inflammation and bone destruction in rheumatoid arthritis
a Effect of SR9009 on the nuclear translocation of Nrf2. Representative fluorescence micrographs of the effect of SR9009 on IL-1β-induced translocation of Nrf2 (green) from three independent experiments (original magnification 20×, scale bar = 100 μm). b RA FLSs were treated with SR9009 at the indicated concentrations for 12 h and stimulated with IL-1β (10 ng/mL) for 30 min. Nuclear extracts of RA FLSs were prepared. Protein levels of p65, Nrf2, GAPDH, and lamin B as determined by western blotting (top) and densitometric analysis of an immunoblot from three independent experiments (bottom). *p < 0.05, **p < 0.01 versus cells stimulated with only IL-1β. c, d RA FLSs were transfected with an NR1D1 siRNA and stimulated with IL-1β for the indicated times. Representative images of the effect of NR1D1 silencing on the phosphorylation of p38, JNK, ERK, p65, IKKα/β, Akt, and IκBα (left). Right panel, densitometric analysis of an immunoblot from three independent experiments (right). *p < 0.05, **p < 0.01 versus cells stimulated with only IL-1β. ns, no significance.
