Fig. 2: Silencing lnc-Lfar1 alleviates CCl4-induced proinflammatory activation of macrophages. | Cell Death & Disease

Fig. 2: Silencing lnc-Lfar1 alleviates CCl4-induced proinflammatory activation of macrophages.

From: Silencing lncRNA Lfar1 alleviates the classical activation and pyoptosis of macrophage in hepatic fibrosis

Fig. 2: Silencing lnc-Lfar1 alleviates CCl4-induced proinflammatory activation of macrophages.

Mice were treated with oil in combination with injection of lenti-NC (NC, n = 10), CCl4 in combination with injection of lenti-NC (NC + CCl4, n = 10), oil in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA, n = 10), and CCl4 in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA + CCl4, n = 10). a Immunohistochemistry analysis was performed to detect the expression of F4/80 and LY6C; scale bar = 400 μm for 10 × and 100 μm for 40×. b The protein level of F4/80, CD11b and LY6C was determined by western blot. GAPDH was used as an internal control. c The mRNA level of F4/80, Ly6c, Ccr2, Cd20, Il-6, iNos, Ccl5, Cxcl5, Cxcl9 and Cxcl10 was determined by qRT-PCR. *p < 0.05 vs NC, #p < 0.05 vs NC + CCl4.

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