Fig. 1: Generation of rpl18 mutation using CRISPR/Cas9 system. | Cell Death & Disease

Fig. 1: Generation of rpl18 mutation using CRISPR/Cas9 system.

From: The nuclear gene rpl18 regulates erythroid maturation via JAK2-STAT3 signaling in zebrafish model of Diamond–Blackfan anemia

Fig. 1

a, b Illustration describing the target site of rpl18. c, d Genomic sequences and predicted protein sequences based on the DNA sequence in wild-type and rpl18−/− mutants. e qRT-PCR of rpl18 transcript levels of wild-type siblings and rpl18−/− mutants at 24 and 48 hpf. qRT-PCR experiments were performed biological repeats and technical repeats in triplicate (N = 3). ***P < 0.001. f WISH results for rpl18 in siblings and mutants at 24 and 48 hpf, WISH results were verified three times independently (N = 3). All scale bars represent 250 μm.

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