Fig. 2: Rpl18-deficient embryos displayed physical abnormalities and anemia. | Cell Death & Disease

Fig. 2: Rpl18-deficient embryos displayed physical abnormalities and anemia.

From: The nuclear gene rpl18 regulates erythroid maturation via JAK2-STAT3 signaling in zebrafish model of Diamond–Blackfan anemia

Fig. 2

a–j Phenotypes of wild-type siblings and rpl18−/− mutants (N = 7). At 24 hpf (a, b), aplasia of head region (dashed box) can be seen in mutant. At 30 hpf (c, d), pigmentation was delayed. At 36 hpf (e, f) and 2 dpf (g, h), a smaller head and edema (arrowhead) were present. At 3 dpf (i, j), edema (arrowhead) in heart and shorted tail extension was more apparent. Almost all embryos died at 4 dpf. k, l o-dianisidine staining showed depletion of erythroid cells in rpl18−/− embryos compared with wild-type siblings (arrowheads pointed to the sites of weak o-dianisidine staining, N = 5). m, n rpl18 mRNA (100 pg/embryo) injected to embryos, staining of 3 dpf mutants with o-dianisidine showed a partial recovery (N = 3). All scale bars represent 250 μm.

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