Fig. 5: Upregulation of p53 activity partially contributed to the erythroid phenotype in rpl18 mutants. | Cell Death & Disease

Fig. 5: Upregulation of p53 activity partially contributed to the erythroid phenotype in rpl18 mutants.

From: The nuclear gene rpl18 regulates erythroid maturation via JAK2-STAT3 signaling in zebrafish model of Diamond–Blackfan anemia

Fig. 5

a Transcript quantification of p53 and p53 related genes were verified by qRT-PCR. All the p53 related genes were highly expressed in rpl18 mutants at 30 hpf (N = 3), *P < 0.05. **P < 0.01. b WISH showed that p53, p21, and mdm2 expression was elevated in rpl18 mutants at 30 hpf (N = 3). c In situ cell death staining result showed that the signal of apoptosis can be observed thought the body at 30 and 48 hpf (N = 4). d An increase of hemoglobin level appeared in mutants at 3 dpf after p53 knockdown (N = 3). Arrowhead points to the recovery of erythrocytes. All scale bars represent 250 μm.

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