Fig. 7: The levels of 5hmC and TET1 were upregulated after hypo-DNAme. | Cell Death & Disease

Fig. 7: The levels of 5hmC and TET1 were upregulated after hypo-DNAme.

From: UHRF1-repressed 5’-hydroxymethylcytosine is essential for the male meiotic prophase I

Fig. 7

a Overexpression of UHRF1, mUHRF1-ΔSRA-FLAG, mUHRF1-ΔTTD-FLAG, and mUHRF1-ΔRING-FLAG plasmids and the level of 5hmC in GC1 cells. b Percentage of the cells with strong or weak intensity of green fluorescence. c Immunohistochemistry assay of DNAme and 5hmC in the mouse spermatocytes. d Venn diagram showing the overlap between hyper-5hmC and hypo-DNAme peaks. The significance was evaluated by Fisher’s exact test. e Immunohistochemistry assay of TET1 in the mouse testis. Scale bar, 10 μm in a, 25 μm in c, e.

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