Fig. 2: Identification of genome-wide transcription targets for SET7/9. | Cell Death & Disease

Fig. 2: Identification of genome-wide transcription targets for SET7/9.

From: SET7/9 promotes multiple malignant processes in breast cancer development via RUNX2 activation and is negatively regulated by TRIM21

Fig. 2: Identification of genome-wide transcription targets for SET7/9.

a ChIP-seq analysis was performed in MCF-7 cells using a specific antibody against SET7/9, and the peaks’ distribution of SET7/9 was determined. b Discriminative regular expression motif elicitation was used to analyze the potential binding motif of SET7/9. c Cellular activities affected by SET7/9 according to Gene Ontology. d Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to identify the pathways in which the potential target genes of SET7/9 were involved. e qChIP analysis was performed in MCF-7 cells using an anti-SET7/9 antibody to detect the binding of SET7/9 to the selected target genes with GAPDH as a negative control. Data are expressed as the fold change over the normal IgG group. Error bars represent the mean ± S.D. for three independent experiments. *P < 0.05; **P < 0.01.

Back to article page