Fig. 2: HIF1A reverses the effect of 1% O2-induced hypoxia on miR-100-5p expression and glycolysis. | Cell Death & Disease

Fig. 2: HIF1A reverses the effect of 1% O2-induced hypoxia on miR-100-5p expression and glycolysis.

From: HIF1A activates the transcription of lncRNA RAET1K to modulate hypoxia-induced glycolysis in hepatocellular carcinoma cells via miR-100-5p

Fig. 2

a HCCLM3 and HepG2 cells were exposed to 1% O2 for 0.5, 3, 6, or 12 h and examined for the protein levels of HIF1A (n = 3). b HIF1A silencing in HCCLM3 and HepG2 cells was achieved by transfection with si-HIF1A#1 or si-HIF1A#2 under 1% O2 exposure, as confirmed by Immunoblotting (n = 3.). HCCLM3 and HepG2 cells were transfected with si-HIF1A, exposed to 1% O2 and examined for c the expression of miR-100-5p (n = 5), d, e lactate concentration and glucose uptake, and f, g the cell viability by BRDU assays. n = 3. *P < 0.05, **P < 0.01, compared to control group; #P < 0.05, ##P < 0.01, compared to 1% O2 group.

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