Fig. 3: PHDMSC-CM reduces apoptosis of intestinal epithelial cells in rats with NEC in vivo and in vitro. | Cell Death & Disease

Fig. 3: PHDMSC-CM reduces apoptosis of intestinal epithelial cells in rats with NEC in vivo and in vitro.

From: Prolyl hydroxylase 2 silencing enhances the paracrine effects of mesenchymal stem cells on necrotizing enterocolitis in an NF-κB-dependent mechanism

Fig. 3

a Apoptosis was assayed by TUNEL staining on day 4 of life (upper panel). Scale bars 100 μm. (lower panel) Quantification of TUNEL-positive cells. n = 3 in each group. The number of positive cells in 5 crypts was scored in 100 crypts per section and reported as mean ± SD. *P < 0.05 vs. NEC + DMEM-F12. #P < 0.05 vs. NEC + MSC-CM. b Apoptosis of IEC-6 was assayed by TUNEL staining 3 days after exposure (upper panel). Scale bars 25 μm. (lower panel) Quantification of TUNEL-positive IEC-6 cells. Data are reported as means ± SD for 10 random fields per wells from four replicate wells per group. **P < 0.05 vs. NEC + DMEM-F12. ##P < 0.05 vs. NEC + MSC-CM. FOV, field of view. c Apoptosis and cell death of IEC-6 were evaluated quantitatively by flow cytometry after PI/Annexin V staining. The right lower quadrant (RLQ) contains early apoptotic cells, and the upper right quadrant (RUQ) contains late apoptotic and necrotic cells. d The percentage of total apoptotic cells and dead cells under each condition from panel C are shown. e The ratio of apoptosis IEC-6 cells was determined by PI/Annexin V staining 1, 3, 5, and 7 days after exposure. Data represent means ± SD of three independent experiments. **P < 0.05 vs. NEC + DMEM-F12.

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