Fig. 5: PHDMSC-CM promotes regeneration of intestinal stem cells in rats with NEC. | Cell Death & Disease

Fig. 5: PHDMSC-CM promotes regeneration of intestinal stem cells in rats with NEC.

From: Prolyl hydroxylase 2 silencing enhances the paracrine effects of mesenchymal stem cells on necrotizing enterocolitis in an NF-κB-dependent mechanism

Fig. 5

a Immunohistochemical staining with Lgr5. Intestinal tissue samples were collected and analyzed on day 4 of life. Scale bars 25 μm. b Quantification of Lgr5-positive cells. n = 3 in each group. The number of positive cells in 5 crypts was scored in 100 crypts per section and reported as mean ± SD. **P < 0.05 vs. NEC + DMEM-F12. ##P < 0.05 vs. NEC + MSC-CM. c The protein levels of Lgr5 in the ileum mucosa of rats were detected by Western blot assays on days 4 and 7 of life with β-actin as the internal control. d Lgr5 mRNA expression in the ileum mucosa of rats after exposure was evaluated by quantitative real-time RT-PCR. β-actin was used as a loading control. Values represent means ± SD; n = 3 in each group. *P < 0.05 vs. NEC + DMEM-F12. #P < 0.05 vs. NEC + MSC-CM.

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